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DUO92103

Sigma-Aldrich

Duolink® In Situ Red Starter Kit Mouse/Goat

Synonym(s):

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

product line

Duolink®

technique(s)

proximity ligation assay: suitable

fluorescence

λex 594 nm; λem 624 nm (red) (Texas Red®; Zeiss Filter set 31)

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), red detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list
Duolink® In Situ Red Starter Kit Mouse/Goat has been used in the critical commercial assay and proximity ligation assay (PLA).

Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

Kit Components Also Available Separately

Product No.
Description
SDS
  • DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)SDS
  • DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUSSDS
  • DUO92008Duolink® In Situ Detection Reagents RedSDS
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS
  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS

Signal Word

Danger

Hazard Statements

H290,H317,H334,H411

Hazard Classifications

Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XIV (Authorisation List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Collaboration between distinct Rab small GTPase trafficking circuits mediates bacterial clearance from the bladder epithelium
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International journal of oncology, 46(6), 2595-2605 (2015-04-23)
Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms, displaying variable morphological and clinicopathological features. Supportive immunohistochemical markers such as CD34, CD99, BCL2 and LSD1 are commonly applied in the differential diagnosis of SFTs, although none is sufficiently sensitive or specific
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Gut, 65(7), 1165-1174 (2015-06-11)
New-onset diabetes and concomitant weight loss occurring several months before the clinical presentation of pancreatic cancer (PC) appear to be paraneoplastic phenomena caused by tumour-secreted products. Our recent findings have shown exosomal adrenomedullin (AM) is important in development of diabetes
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Protocols

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

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