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D9656

Sigma-Aldrich

Anti-DNP antibody produced in rabbit

whole antiserum

Synonym(s):

Anti Dnp, Anti Dnp Antibody, Anti-DNP - Anti-DNP antibody produced in rabbit, Anti-Dnp, Anti-Dnp Antibody, Dnp Antibody

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About This Item

MDL number:
MFCD00162263
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:8,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Immunogen

A DNP-BSA conjugate

Application

Anti-DNP antibody produced in rabbit was used to tag derivatized protein carbonyl groups in plasma by ELISA. It was used for differential cell count of zona-free blastocysts in IVF embryos.

Biochem/physiol Actions

2,4-Dinitrophenyl is an oxidative phosphorylation inhibitor and hinders adenosine triphosphate (ATP) synthesis. Due to its low excretion rate, 2,4-dinitrophenyl is considered toxic to human. It increases electron transportation, respiration and overall metabolism.

Analysis Note

Antiserum is tested for specificity and titer by immunoelectrophoresis; treated to remove lipoproteins..

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael Hoelker et al.
Reproduction (Cambridge, England), 137(3), 415-425 (2008-12-23)
The Well of the Well (WOW) system has been developed to culture embryos in small groups or to track the development of single embryos. In the present study, we aimed to examine the effects of the microenvironment provided by the
Liliana Matos et al.
Oxidative medicine and cellular longevity, 2017, 9172085-9172085 (2017-11-18)
[This corrects the article DOI: 10.1155/2017/3793817.].
Biochemistry Biochemistry: Solutions Manual, 49-49 (1998)
Athanassios Fragoulis et al.
Redox biology, 12, 843-853 (2017-04-28)
There is increasing evidence for the involvement of chronic inflammation and oxidative stress in the pathogenesis of Alzheimer's disease (AD). Nuclear factor erythroid 2-related factor 2 (Nrf2) is an anti-inflammatory transcription factor that regulates the oxidative stress defense. Our previous
Evelyn I Back et al.
The American journal of clinical nutrition, 80(2), 374-384 (2004-07-28)
Little is known about age- and disease-related changes in prooxidant and antioxidant systems in patients with cystic fibrosis (CF). We investigated changes in antioxidant concentrations and oxidative stress in plasma, buccal mucosal cells (BMCs), and breath condensate in patients with
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