D8899
Deoxyribonucleic acid, single stranded from calf thymus
lyophilized powder
Synonym(s):
DNA single stranded
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
grade
for molecular biology
Quality Level
form
lyophilized powder
mol wt
~50 kb
composition
ssDNA, ≥65%
storage temp.
−20°C
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General description
High quality single-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Features and Benefits
• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
Quantity
One mg DNA is approx. 25 A260 units.
Preparation Note
Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.
Reconstitution
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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