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D8144

Sigma-Aldrich

Dextranase from Penicillium sp.

lyophilized powder, 400-800 units/mg protein

Synonym(s):

1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Penicillium sp.

Quality Level

form

lyophilized powder

specific activity

400-800 units/mg protein

composition

Protein, 35% Lowry

storage temp.

2-8°C

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General description

Dextranase from fungi belong to the glycoside hydrolase families (GH) 49. Dextranase from Penicillium sp. are stable at varying temperature and pH.

Application

Dextranase from Penicillium sp has been used:
  • in the digestion of exopolysaccharides from P. aeruginosa biofilms and dextran polymers
  • as a component of trypticase soy broth (TSYPS) for Streptococcus mutans culture
  • in microgel degradation

Biochem/physiol Actions

An endodextranase that hydrolyzes -(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production that increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Unit Definition

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Label-free high-throughput functional lytic assays
O'Malley SM, et al.
Journal of Biomolecular Screening, 12(1), 117-125 (2007)
Biology and genome sequence of Streptococcus mutans phage M102AD
Delisle AL, et al.
Applied and Environmental Microbiology, 78(7), 2264-2271 (2012)
T I Danilova et al.
Prikladnaia biokhimiia i mikrobiologiia, 19(1), 104-110 (1983-01-01)
The carbohydrate composition of dextranase from Penicillium funiculosum 15, as well as the composition of products of dextran deep hydrolysis by the enzyme were studied. The products are normally used to stabilize the enzyme during its purification. Using the methods
Dextranase from Penicillium minioluteum: reaction course, crystal structure, and product complex
Larsson AM, et al.
Structure, 11(9), 1111-1121 (2003)
Hordenine: a novel quorum sensing inhibitor and antibiofilm agent against Pseudomonas aeruginosa
Zhou JW, et al.
Journal of Agricultural and Food Chemistry, 66(7), 1620-1628 (2018)

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