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D6815

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

peroxidase substrate, liquid, suitable for membrane applications

Synonym(s):

DAB membrane substrate

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About This Item

MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24894054
NACRES:
NA.83

product name

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride, for Membrane Applications

Quality Level

200

form

liquid

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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Application

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
  • in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining
  • in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi
  • in immunoperoxidase staining of lung tissue
The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

Biochem/physiol Actions

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.

Packaging

Set consists of 4 x 100 ml Solution A and 15 ml Solution B.

Caution

Stable for up to 18 mo. when stored at 2-8 °C in the original container.

Quantity

Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

Kit Components Also Available Separately

Product No.
Description
SDS
  • 3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H341,H350

Hazard Classifications

Carc. 1B - Muta. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Preclinical efficacy studies of influenza A haemagglutinin precursor cleavage loop peptides as a potential vaccine
Miller DS, et al.
The Journal of General Virology, 92, 1152-1161 (2011)
Preclinical efficacy studies of influenza A haemagglutinin precursor cleavage loop peptides as a potential vaccine
Miller DS, et al.
The Journal of General Virology, 92(5), 1152-1161 (2011)
Acute erythropoietin cardioprotection is mediated by endothelial response
Teng R, et al.
Basic Research in Cardiology, 106, 343-354 (2011)
Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region
Nieves E, et al.
Journal of Vector Borne Diseases, 49(1), 8-8 (2012)
Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region
Nieves E, et al.
Journal of Vector Borne Diseases, 49, 8-8 null
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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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