OE33 Cell Line human

Caucasian esophageal carcinoma, 96070808

• Synonym(s): JROECL33, JROECL33 Cells, OE-33 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human esophagus
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Epithelial
• products: Not specified
• receptors: MHC class I, ICAM-1
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian oesophageal carcinoma

Cell Line Description

The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

Application

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.

Study of oesophageal cancer

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

Other Notes

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