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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-Yellow Fever Virus
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a, kappa
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against the envelope protein of the wild type and vaccine strains of yellow fever virus. It does not cross react with other flaviviruses.
Immunogen
Yellow fever virus, strain 17D
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-mercaptoethanol (2ME) + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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