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Key Documents

KLN 205

90110519

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About This Item

UNSPSC Code:
41106514

biological source

mouse unknown/unspecified

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse squamous cell carcinoma

Cell Line Description

Cells produce metastases in the lungs when inoculated intravenously into DBA/2 or BDF1 mice. The cells are a potential model for chemotherapeutic screening.

Application

Chemotherapeutic agent and tumourigenicity studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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