Recommended Products
product name
N18TG2, 08062523
biological source
mouse nerve
growth mode
Adherent
karyotype
mean chromosome number is 89
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse neuroblastoma
Cell Line Description
A 6-thioguanine-resistant clone of N18 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line exhibits high levels of acetylcholinesterase. Found to be tumourigenic in BALB/c nude mice.
Application
Used in the generation of cell hybrids.
Culture Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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