C2780
Chondroitinase AC from Flavobacterium heparinum
lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
Synonym(s):
Chondroitin AC lyase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
conjugate
(Glucosaminoglycan)
Quality Level
form
lyophilized powder
specific activity
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
composition
Protein, ~15% Lowry
solubility
0.02 M phosphate buffer: soluble (pH 7.0)
foreign activity
Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
storage temp.
−20°C
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Application
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Biochem/physiol Actions
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Other Notes
Contains potassium phosphate buffer salts and BSA as stabilizer.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Suitability
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
Unit Definition
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
Preparation Note
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Florian D Naal et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 87(1), 19-25 (2008-03-25)
Meniscal allograft processing procedures, in particular gamma irradiation, deteriorate the biomechanical and biological properties of the transplanted tissue. High hydrostatic pressure (HHP) treatment, widely used in food technology to inactivate microorganisms while preserving natural compounds, might serve as a gentle
Jessica S Kim et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(8), 780-790 (2011-08-02)
Cutaneous lupus erythematosus and dermatomyositis (DM) are chronic inflammatory diseases of the skin with accumulated dermal mucin. Earlier work has shown chondroitin sulfate (CS) accumulation within the dermis of discoid lupus erythematosus (DLE), subacute cutaneous lupus erythematosus (SCLE), and DM
E B Hunziker
Osteoarthritis and cartilage, 9(1), 22-32 (2001-02-17)
We have previously shown (Hunziker and Rosenberg, J Bone Joint Surg 1996;78A:721-33) that synovial cells can be induced to migrate into partial-thickness articular cartilage defects, therein to proliferate and subsequently to deposit a scar-like tissue. We now wished to ascertain
C B Knudson et al.
The Journal of cell biology, 100(5), 1753-1758 (1985-05-01)
Hyaluronate levels change dramatically during morphogenesis of various tissues and organs. Morphological detection of the exact temporal and spatial distribution patterns of hyaluronate may help to elucidate its role in morphogenesis. Since no specific direct method for visualizing hyaluronate with
S P Levine et al.
Blood, 75(4), 902-910 (1990-02-15)
Platelet factor 4 (PF4) is a hydrophobic, alpha-granule protein with potent antiheparin activity. It also binds to a chondroitin sulfate-containing proteoglycan (PG) isolated from platelets. In order to evaluate further the relationship between PF4 and the chondroitin sulfate-containing proteoglycan in
Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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