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54327

Sigma-Aldrich

Lipase, immobilized on Immobead 150 from Pseudomonas cepacia

≥900 U/g

Synonym(s):

Immobilized Lipase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Pseudomonas cepacia)

Quality Level

100

form

powder (or beads)

specific activity

≥900 U/g

color

white to off-white

application(s)

life science and biopharma

storage temp.

2-8°C

Gene Information

Burkholderia spp. ... BURCE16_RS29975(56665765)

General description

Research area: Cell Signaling

Lipases are water-soluble, ester hydrolases. Long aliphatic chain acyl esters of cholesterol (cholesteryl esters), triacyl esters of glycerol (triacylglycerols), acyl esters of long chain alcohols (wax esters), diacyl esters of glycerol (diacylglycerols), and monoacyl esters of glycerol, are some of the common substrates for lipases.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.Lipase, immobilized on Immobead 150 from Pseudomonas cepacia has been used in enzymatic hydrolysis of polylactic acid (PLA). It has also been used as biocatalysts in lipase-catalyzed kinetic resolution reactions.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.Lipases are known to regulate energy metabolism of the cell. Lipolysis is highly essential for cellular uptake or release of fatty acids (FAs) and glycerol. Gastrointestinal lipolysis catabolizes dietary fat. Vascular lipolysis hydrolyzes lipoprotein-related triglycerides (LTPs) in the blood. In the intracellular domain, lipolysis catalyzes the breakdown of triglycerides (TGs) stored in LDLs for exportation of fatty acids (fat) (fat tissue) or metabolism (non-adipose tissue).

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Florian Golombek et al.
ACS omega, 6(43), 29192-29200 (2021-11-09)
Deracemizations are clearly preferable to kinetic resolutions in the production of chiral molecules from racemates, as they allow up to 100% chemical and optical yield. Here we present a new process route for multienzymatic deracemizations that is relevant for reaction
Song-Hua Hu et al.
Cell reports, 38(11), 110509-110509 (2022-03-17)
Protein fatty acylation regulates numerous cell signaling pathways. Polyunsaturated fatty acids (PUFAs) exert a plethora of physiological effects, including cell signaling regulation, with underlying mechanisms to be fully understood. Herein, we report that docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)
Guan-Chun Chen et al.
Biotechnology letters, 33(3), 525-529 (2010-11-04)
Triacylglycerol lipase from Pseudomonas cepacia and Fe(3)O(4) magnetic nanoparticles were encapsulated simultaneously within biomimetic silica through the catalysis of polyallylamine. The encapsulation efficiency reached 96% with an activity recovery of 51%. After 5 h at 37°C, the activities of the
Industrial applications of microbial lipases
F. Hasan et al.
Enzyme and Microbial Technology, 39, 235-251 (2006)

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