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51435C

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Dulbecco′s Modified Eagle′s Medium/High Modified

DMEM/High Modified, with 4500 mg/L dextrose, with 110 mg/L sodium pyruvate, without ferric nitrate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

components

L-glutamine: no
glucose: high
phenol red: yes
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Dulbecco′s Modified Eagle′s Medium has high concentration of amino acids and vitamins.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s modified eagle′s medium/high modified has been used in the characterisation of nanoparticles in cell culture media.
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Cancer Cell Culture: Methods and Protocols (2004)
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Human adipose stem cells can differentiate into various mesodermic lineages, including adipogenic, osteogenic, chondrogenic, myogenic and endothelial pathways. In addition, these cells types possess immunomodulatory properties, potentially useful for autoimmune and autoinflammatory diseases. However, single-cell expanded clones have shown that
Seetharam Deepa et al.
Theriogenology, 161, 161-175 (2020-12-18)
Wnt signaling is conserved among all species and plays a significant role in various cellular processes including reproduction. The present study identified significant involvement of wnt4a, wnt5b, and wnt8a signaling in the testicular growth of common carp,Cyprinus carpio. Predominant expression

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