32922

Lugol solution

according to Lugol

• Synonym(s): Iodine/Potassium iodide solution
• MDL number: MFCD00134128
• UNSPSC Code: 12171500
• PubChem Substance ID: 329754388
• NACRES: MA.02

Properties

• form: liquid
• Quality Level: 200
• quality: according to Lugol
• density: 1.006 g/cm³ at 20 °C
• application(s): diagnostic assay manufacturing; food and beverages; hematology; histology
• storage temp.: room temp
• SMILES string: [K+].[I-].II
• InChI: 1S/I2.HI.K/c1-2;;/h;1H;/q;;+1/p-1
• InChI key: XUEKKCJEQVGHQZ-UHFFFAOYSA-M

Description

General description

Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology. It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.

Application

• Lugol′s solution is generally used as a mordant in Gram staining, and for fixing invertebrate blood cells and protozoa.
• It is suitable for the detection of starch in plants.
• It has been used in a study to assess narrow-band imaging without image magnification for detecting high-grade dysplasia and intramucosal esophageal squamous cell carcinoma.
• It has been used in a study to determine its benefit in aiding the detection, presence, and spread of small squamous cell carcinomas of the esophagus.

Biochem/physiol Actions

Iodine diffuses rapidly through hydrophilic and hydrophobic domains of tissues and loosely binds to proteins, staining them brown. It reacts irreversibly with unsaturated lipid linkages and with tyrosine and histidine aromatic rings.

Features and Benefits

• Ready-to-use solution.

Principle

In bacteriology, Gram staining allows rapid differentiation between Gram-positive and Gram-negative bacteria. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with the primary stain, crystal violet. Lugol solution is added thereafter as the mordant, forming crystal violet-iodine complexes. During the decolorization, these complexes stay entrapped in the multilayer mureine structure of the cell wall of Gram-positive bacteria which appear blue-violet. Gram-negative bacteria have a single-layered cell wall that does not retain the dye-iodine complexes and are counterstained with safranine solution, appearing pink.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves