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D2535

Sigma-Aldrich

Direct Blue 15

suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

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About This Item

Linear Formula:
C34H24N6O16S4Na4
CAS Number:
2429-74-5
Molecular Weight:
992.80
Colour Index Number:
24400
EC Number:
219-385-3
MDL number:
MFCD00036441
UNSPSC Code:
12171500
PubChem Substance ID:
24893697
NACRES:
NA.47

description

suitable for Histopaque® system

Quality Level

200

form

powder

composition

Dye content, ~40%

solubility

water: 10 mg/mL, clear, blue

suitability

suitable for viability studies of collagenase-treated rat liver cells

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].COc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(OC)c4

InChI

1S/C34H28N6O16S4.4Na/c1-55-25-9-15(3-5-23(25)37-39-31-27(59(49,50)51)11-17-7-19(57(43,44)45)13-21(35)29(17)33(31)41)16-4-6-24(26(10-16)56-2)38-40-32-28(60(52,53)54)12-18-8-20(58(46,47)48)14-22(36)30(18)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

OLSOUGWNONTDCK-UHFFFAOYSA-J

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Application

May be used as a substitute for trypan blue for some biological and staining applications.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
015F00601

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A Kai et al.
International journal of biological macromolecules, 20(3), 221-231 (1997-06-01)
The difference of influence of a certain kind of direct dye on the structure of nascent microbial cellulose was examined, with Direct Red 28 have a biphenylenebis(azo) skeletal structure; Direct Blue 1 having two hydroxyl, two methoxy and two sulfonate
A Shan et al.
Journal of chromatographic science, 26(9), 439-442 (1988-09-01)
Two structurally similar azo dyes, Direct Blue No. 6 and Direct Blue No. 15, are analyzed by separate reversed-phase high-performance liquid chromatographic (HPLC) systems utilizing a dual-wavelength UV/visible detector at 254 and 546 nm. Each dye contains more than 35
Metabolism of benzidine and benzidine-congener based dyes by human, monkey and rat intestinal bacteria.
C E Cerniglia et al.
Biochemical and biophysical research communications, 107(4), 1224-1229 (1982-08-31)
Koel Kumar et al.
Bioresource technology, 98(16), 3168-3171 (2007-02-27)
Studies were carried out on decolorisation and biotransformation of the dye Direct Blue-15 into 3,3'-dimethoxybenzidine (O'-dianisidine) and a sulphonated derivative by a five-member bacterial consortium. Chromatographic studies revealed further complete biodegradation of 3,3'-dimethoxybenzidine coupled with release of ammonia, but the
Chih-Huang Weng et al.
Ultrasonics sonochemistry, 20(3), 970-977 (2012-11-29)
Decolourization of direct azo dye, direct blue 15 (DB15), by an advanced Fenton process coupled with ultrasonic irradiation (Fenton/US) was investigated. Zero-valent iron (ZVI) aggregates were used as the catalyst. A positive synergistic effect occurred when Fenton's reagent was combined
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