66721
Methylene Blue zinc chloride double salt
for microscopy (Bact., Bot., Hist.), ~1 mol/mol water
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S · 0.5ZnCl2 · xH2O
CAS Number:
Molecular Weight:
388.00 (anhydrous basis)
Beilstein:
3839953
MDL number:
UNSPSC Code:
12352106
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
grade
for microscopy (Bact., Bot., Hist.)
Quality Level
impurities
~1 mol/mol water
SMILES string
O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI
1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChI key
WQVSELLRAGBDLX-UHFFFAOYSA-M
Application
Methylene blue is a heterocyclic aromatic chemical compound used as a cationic dye. It is used as a redox indication in analytical chemistry. Methylene blue is a component of a number of staining protocols such as Wright′s stain and Jenner′s stain for microscopic evaluation.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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K P Krafts et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 86(1), 7-35 (2011-01-18)
The components of the blood stain, eosin and methylene blue, were introduced by Baeyer and Caro, respectively. Methylene blue was used primarily for detecting Mycobacterium tuberculosis until Ehrlich in 1880 mixed methylene blue with acid fuchsin to produce what he
A Van Deun et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(12), 1571-1575 (2010-12-15)
Damien Foundation Bangladesh tuberculosis (TB) control projects. To compare blue ink, potassium permanganate and methylene blue background staining for transmitted light-emitting diode (LED) TB fluorescence microscopy (FM). Auramine smears made in triplicate from Ziehl-Neelsen (ZN) acid-fast bacilli (AFB) positive or
Kristine Krafts et al.
Parasitology research, 109(3), 521-529 (2011-06-11)
Methylene blue was synthesized by Caro in 1876 at BASF, a chemical company. Six years later, Koch employed methylene blue when he discovered the tubercle bacillus. In 1880, Ehrlich described what he termed "neutral" dyes: mixtures of acidic and basic
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