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17153

Millipore

LS Differential Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Lactobacillus Streptococcus Differential Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
beef extract, 5 g/L
casein enzymic hydrolysate, 10 g/L
L-cysteine hydrochloride, 0.3 g/L
dextrose, 20 g/L
papaic digest of soybean meal, 5 g/L
sodium chloride, 5 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.1±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Lactobacillus spp.
nonselective and differential for Streptococcus spp.

Related Categories

Application

For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, T.T.C. reduction and casein reaction.

Preparation Note

Suspend 65.3 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the following sterile solutions previously kept warm at 50°C just prior to use; (1) 100 ml of 10% (w/v) aqueous solution of antibiotic free skim milk powder (Cat. No. No. 70166) sterilized at 121°C for 5 minutes.(2) 10 ml of TTC Solution (Cat. No. 17779). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG3238
BCCB5483
BCCB0217
BCBR7648V
BCBN8877V

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Articles

Microbiological Media and Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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