MABE872
Anti-Histone H3.3 Antibody, clone 4H2D7
clone 4H2D7, 1 mg/mL, from rat
Synonym(s):
Histone H3.3, H3F3A, H3.3A, H3F3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4H2D7, monoclonal
species reactivity
mouse, human
concentration
1 mg/mL
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H3F3B(3021)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3.3.
Application
Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected Histone H3.3 in HeLa cells.
Western Blotting Analysis: A representative lot from an independent laboratory detected recombinant Histone H3.3 (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.3 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Western Blotting Analysis: A representative lot from an independent laboratory detected recombinant Histone H3.3 (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Histone H3.3 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.3 from C2C12 cell lysate (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This Anti-Histone H3.3 Antibody, clone 4H2D7 is validated for use in western blotting, ICC & ChIP for the detection of Histone H3.3.
Quality
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.
Western Blotting Analysis: 2 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.
Target description
~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rebecca L Murdaugh et al.
Stem cell reports, 16(8), 2014-2028 (2021-07-10)
Histone variants contribute to the complexity of the chromatin landscape and play an integral role in defining DNA domains and regulating gene expression. The histone H3 variant H3.3 is incorporated into genic elements independent of DNA replication by its chaperone
Akihito Harada et al.
The EMBO journal, 31(13), 2994-3007 (2012-05-10)
Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of
Guang Yang et al.
Nucleic acids research, 48(6), 3001-3013 (2020-01-23)
Nucleosomal histones are barriers to the DNA repair process particularly at DNA double-strand breaks (DSBs). However, the molecular mechanism by which these histone barriers are removed from the sites of DNA damage remains elusive. Here, we have generated a single
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