HPRTKMAG-01K

MILLIPLEX^® RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: ambient

Description

General description

RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in human genome, approximately 60 are members of the RTK family. They are central components of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.

The MILLIPLEX^® RTK Phosphoprotein Bead Customizable Panel is used to detect changes in phosphorylated (panTyr) c-Kit, c-Met/HGFR, EGFR, ErbB2/HER2, ErbB3/HER3 , ErbB4/HER4 , FGFR1 , FLT3 , IGF-1R, M-CSFR, PDGFRα, PDGFRβ, TIE1, TIE2, VEGFR1, VEGFR2, and VEGFR3 in cell lysates using the Luminex^® system.

The MILLIPLEX^® portfolio offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition, each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility.

The MILLIPLEX^® RTK Phosphoprotein Bead Panel includes:

• Positive and negative control cell lysates provided to qualify assay performance
• Configurable beads to capture analytes of interest
• Optimized detection antibody cocktail designed to yield consistent analyte profiles within a panel

Panel Type: Cell Signaling

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

• Analytes: c-Kit, c-Met/HGFR (panTyr), EGFR (pan Tyr), ErbB2/HER2, ErbB3/HER3 (panTyr) ,ErbB4/HER4 (panTyr), FLT3 (pan Tyr), IGF-1R (pan Tyr), M-CSFR (pan Tyr), PDGFR-α (pan Tyr), PDGFRß (pan Tyr), TIE1 (pan Tyr,) Tie2 (pan Tyr), VEGFR1 (pan Tyr), VEGFR2 (pan Tyr), VEGFR3 (pan Tyr), FGFR1
• This 17-plex panel, unlike most signaling kits that are fixed/premixed, is customizable so that it is possible to choose only those analytes that are wanted or needed.
• Recommended Sample type: cell lysate
• Recommended starting concentration: 40 to 1,000 μg protein/mL
• Assay Run Time: Overnight
• Research Category: Signaling Apoptosis & Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96-well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318 - H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3