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Sigma-Aldrich

Vitronectin, Human Purified Protein

Synonym(s):

S-protein

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

Assay

≥90% (SDS-PAGE)

form

liquid

specific activity

1 μg/mL (cell attachment assays)

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... VTN(7448)

Related Categories

General description

PROPERTIES:

Binds to heparin. Active in cell attachment of MG63 cells.
Product Source: Human plasma; tested negative for antibodies to HBsAg and HIV.
Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.

Application

Suitable for use in ELISA, immunization, cell attachment assays and electrophoresis.

Linkage

Replaces: 08-126

Physical form

Liquid in 50 mM sodium phosphate, 100 mM NaCl, pH 7.4, containing no preservatives.

Storage and Stability

Stable 12 months at -70°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analysis Note

Two bands in SDS-PAGE at 65 kDa and 75 kDa.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Roman M Salasznyk et al.
Experimental cell research, 313(1), 22-37 (2006-11-04)
The intracellular signaling events controlling human mesenchymal stem cells (hMSC) differentiation into osteoblasts are not entirely understood. We recently demonstrated that contact with extracellular matrix (ECM) proteins is sufficient to induce osteogenic differentiation of hMSC through an ERK-dependent pathway. We
Alan Tin-Lun Lam et al.
Stem cells and development, 23(14), 1688-1703 (2014-03-20)
The expansion of human pluripotent stem cells (hPSC) for biomedical applications generally compels a defined, reliable, and scalable platform. Bioreactors offer a three-dimensional culture environment that relies on the implementation of microcarriers (MC), as supports for cell anchorage and their
Andrew J Putnam et al.
Cell communication and signaling : CCS, 7, 10-10 (2009-04-30)
Integrins, cell-surface receptors that mediate adhesive interactions between cells and the extracellular matrix (ECM), play an important role in cancer progression. Expression of the vitronectin receptor alphavbeta3 integrin correlates with increased invasive and metastatic capacity of malignant melanomas, yet it

Articles

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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