AP502B
Goat Anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed
0.5 mg/mL, Chemicon®
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About This Item
Recommended Products
biological source
goat
Quality Level
conjugate
biotin conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
concentration
0.5 mg/mL
technique(s)
ELISA: suitable
immunofluorescence: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Specificity
Reacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains and mouse immunoglobulins.
The antibody reacts with human kappa light chains as demonstrated by ELISA and flow cytometry.
Immunogen
Epitope: Ig kappa chain
Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with κ light chains.
Application
Immunofluorescence: ≤ 1 μg/106 cells.
ELISA: 1:5,000-1:20,000.
Optimal working dilutions must be determined by the end user.
ELISA: 1:5,000-1:20,000.
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
This Goat anti-Human Ig κ chain Antibody, biotin conjugate, Species Adsorbed is validated for use in ELISA, IF for the detection of Human Ig κ chain.
Physical form
Affinity Chromatography
Purified by affinity chromatography on pooled human Igs with κ light chains covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.
Storage and Stability
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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