AB1541
Anti-Tryptophan Hydroxylase Antibody
Chemicon®, from sheep
Synonym(s):
Tryptophan 5-monooxygenase 1, indoleacetic acid-5-hydroxylase, tryptophan 5-hydroxylase, tryptophan 5-monooxygenase, tryptophan hydroxylase (tryptophan 5-monooxygenase), tryptophan hydroxylase 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
sheep
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... TPH1(7166)
General description
Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.
Specificity
Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species.
Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.
Immunogen
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Application
Detect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.
Immunohistochemistry(peroxidase):
Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.
Optimal working dilutions must be determined by the end user.
Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.
Optimal working dilutions must be determined by the end user.
Quality
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
Target description
55 kDa
Linkage
Replaces: AB1556
Physical form
Purified sheep polyclonal liquid in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 μg/mL BSA and 50% glycerol.
Storage and Stability
Stable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Control
Brain tissue
PC12 lysates
Brain tissue
PC12 lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
S J Bang et al.
Neuroscience, 179, 1-8 (2011-02-01)
Adolescence is a critical vulnerable period during which exposure to nicotine greatly enhances the possibility to develop drug addiction. Growing evidence suggests that serotonergic (5-HT) neurotransmission may contribute to the initiation and maintenance of addictive behavior. As the dorsal raphe
Extrasynaptic glycine receptors of rodent dorsal raphe serotonergic neurons: a sensitive target for ethanol.
Maguire, EP; Mitchell, EA; Greig, SJ; Corteen, N; Balfour, DJ; Swinny, JD; Lambert, JJ; Belelli, D
Neuropsychopharmacology null
Neural connections of the anterior hypothalamus and agonistic behavior in golden hamsters.
Y Delville, G J De Vries, C F Ferris
Brain, behavior and evolution null
Reduction of serotonergic neurons in the dorsal raphe due to chronic prenatal administration of a tryptophan-free diet.
Guadalupe M Flores-Cruz,Alfonso Escobar
International Journal of Developmental Neuroscience null
The activation of raphe serotonergic neurons in normal and hypoarousal-driven aggression: a double labeling study in rats.
Jozsef Haller, Mate Toth, Jozsef Halasz
Behavioural Brain Research null
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