539131
Protease Inhibitor Cocktail I
lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases
Synonym(s):
Protease Inhibitor Mix, Protease inhibitor
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.28
Recommended Products
product name
Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
shipped in
wet ice
storage temp.
−20°C
General description
Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.
Specificity
Inhibits a broad range of proteases and esterases.
Application
Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
- cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
- RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
- sucrose solution to homogenize mice cortices for myelin isolation
Biochem/physiol Actions
Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.
Features and Benefits
- Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
- Ready for reconstitution
Warning
Toxicity: Harmful (C)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.
Other Notes
The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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