524618

PIP3 Antagonist, PITenin-7

The PIP3 Antagonist, PITenin-7 controls the biological activity of PIP3.

• Synonym(s): PIP3 Antagonist, PITenin-7, N-(4-Hydroxybiphenyl-3-ylcarbamothioyl)-3,5-dimethylbenzamide, Phosphatidylinositol-3,4,5-triphosphate Antagonist, PITenin-7, PIT-7, Akt Inhibitor XX, PDK1 Inhibitor V
• Empirical Formula (Hill Notation): C₂₂H₂₀N₂O₂S
• CAS Number: 1356060-65-5
• Molecular Weight: 376.47
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: semisolid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow-white
• solubility: DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable DM-PIT-1 (Cat. No. 524619) analog that is more selective and potent than DM-PIT-1 against PH domain-mediated PIP3 (Cat. No. 524615) binding to Akt1 (IC₅₀ = 13.4 and 27.1 µM, respectively) and PDK1 (IC₅₀ = 52.3 and 80.5 µM, respectively), while being much less active than DM-PIT-1 against PIP3 GRP1 PH domain binding (12% vs 84% inhibition with 100 µM respective compound). PIT-7 is also shown to be more effective than DM-PIT-1 in inhibiting human glioblastoma U87MG survival (IC₅₀ = 6.6 vs. 33.9 µM, respectively) and cellular Akt Ser473 phosphorylation in cultures in vitro.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Miao, B., et al. 2010. Proc. Natl. Acad. Sci. USA107, 20126.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable