182750

ASB-14

A zwitterionic amidosulfobetaine detergent useful for solubilizing proteins visualized by 2D-electrophoresis.

• Synonym(s): ASB-14
• Empirical Formula (Hill Notation): C₂₂H₄₆N₂O₄S
• Molecular Weight: 434.68
• UNSPSC Code: 12161902

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white to off-white
• solubility: water: 50 mg/mL
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

A zwitterionic amidosulfobetaine detergent that exhibits better protein extraction properties than CHAPS.

A zwitterionic amidosulfobetaine detergent useful for solubilizing proteins visualized by 2D-electrophoresis. The increased solubilization produced by this detergent enables the identification of previously undetected membrane proteins.

Application

• Effect of bovine pericardial extracellular matrix scaffold niche on seeded human mesenchymal stem cell function.: This paper explores the influence of the bovine pericardial extracellular matrix on human mesenchymal stem cells, with ASB-14 used to modulate cellular responses. The research contributes to advancements in scaffold-based tissue engineering (Liu et al., 2016).


• In vivo xenogeneic scaffold fate is determined by residual antigenicity and extracellular matrix preservation.: Investigating the fate of xenogeneic scaffolds in vivo, this study shows that ASB-14 helps maintain extracellular matrix integrity and reduce antigenicity, which is critical for successful scaffold integration and function in tissue engineering (Wong et al., 2016).

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Other Notes

Carroll, J., et al. J. Biol. Chem.277, 50311.
Herbert, B. 1999. Electrophoresis20, 660.
Chevallet, M. et al. 1998. Electrophoresis 19, 1901.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable