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Key Documents

14-782-M

Sigma-Aldrich

PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.

Synonym(s):

Active Kinase Protein, Human PI 3-Kinase

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100
300

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 125.3 kDa (p110α; E542K)
Mw 83.7 kDa (p85α)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_006218.2

UniProt accession no.

P42336

Gene Information

human ... PIK3CA(5290)

General description

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K and untagged, recombinant, full-length human p85α.
The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.

Application

Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.

Packaging

Also available in 250 μg size --call for pricing and availability and reference catalog number 14-782M when ordering the 250 μg size.

Storage and Stability

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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