1.01167

Amido black 10 B (C.I.20470)

for electrophoresis

• Synonym(s): Amido black 10 B (C.I.20470), Black acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black
• Empirical Formula (Hill Notation): C₂₂H₁₄N₆Na₂O₉S₂
• CAS Number: 1064-48-8
• Molecular Weight: 616.49
• MDL number: MFCD00004017
• UNSPSC Code: 41105305
• EC Index Number: 213-903-1

Properties

• Quality Level: 400
• form: solid
• potency: >5000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥80% spectrophotometric assay
• pH: 9.0 (25 °C, 10 g/L in H₂O)
• solubility: 10 g/L
• bulk density: 670 kg/m³
• εmax: 750-960 at 617-620 nm in water at 0.01 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,24H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25?,27-21+;;
• InChI key: HKBVRFLHNUEVRO-DWTBGCDMSA-L

Description

General description

Amido black 10 B (C.I. 20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 617 - 620 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; water): 750 - 960
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for electrophoresis: passes test
Suitability as redox indicator: passes test

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(C): Not applicable