09-881

Anti-acetyl Cortactin Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Amplaxin, Oncogene EMS1, Src substrate cortactin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): Drosophila (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_005222.2
• UniProt accession no.: Q14247
• shipped in: wet ice
• target post-translational modification: acetylation (not specified)
• Gene Information: human ... CTTN(2017)

Description

General description

Cortactin is a cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

Recent research efforts show cortactin is acetylated in vivo and is deacetylated by HDAC6 (Histone deacetylase 6). HDAC6 is a tubulin-specific deacetylase that regulates microtubule-dependent cell movement. In recent studies it was demonstrated that HDAC6 binds cortactin and alters the ability of cortactin to bind F-actin by modulating a “charge patch” in its repeat region. Findings suggest that, in addition to its role in microtubule-dependent cell motility, HDAC6 influences actin-dependent cell motility by altering the acetylation status of cortactin, which, in turn, changes the F-actin binding activity of cortactin. The acetylation level of cortactin may correlate to tumor metastases and acetylated cortactin may be considered a potential metastasis marker.

Specificity

The antibody recognizes human acetylated Cortactin.

Immunogen

Consensus sequence motif of acetylation sites of cortactin.

Epitope: Unknown

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-acetyl Cortactin Antibody is validated for use in Western Blotting, for the detection of acetyl Cortactin.

Quality

Evaluated by Western Blot in HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

Western Blot Analysis: 2.5 µg/ml of this antibody detected Cortactin on 10 µL of HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide.

Target description

~ 85 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable