G006032
HPLC Analysis MAb Variants on Proteomix® SCX-NP5, Fast Analysis
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164)
mobile phase: [A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5
gradient: top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min
flow rate: 0.8 mL/min or 1.5 mL/min
detector: UV 280 nm
injection: 20 μL
sample: MAb 321, 5 mg/mL
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
Legal Information
analytical column
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