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G004733

HPLC Analysis of Ritalinic Acid and Ritalin (Methylphenidate) Enantiomers on Astec® CHIROBIOTIC® V2 with T2 Guard Column

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T2 guard column, 2 cm x 4 mm I.D., 5 μm particles (16100AST (requires holder 21150AST))
column: CHIROBIOTIC V2, 15 cm x 4.6 mm I.D., 5 μm particles (15023AST)
mobile phase: [A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (7:93, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 2 μL
sample: 1 mg/mL in methanol

suitability

application for HPLC

application(s)

forensics and toxicology
pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

analytical column

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