50881-U

Ascentis^® Express F5, 2 μm UHPLC Column

2 μm particle size, L × I.D. 15 cm × 3 mm

• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 1000 bar max. pressure (14500 psi); 60 °C temp. range
• technique(s): LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 15 cm × 3 mm
• surface area: 120 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 2 μm
• pore size: 90 Å pore size
• operating pH: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Ascentis^® Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core^® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis^® Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis^® Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

UHPLC/MS Analysis of Mycotoxins on Ascentis^® Express F5, 2 μm

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.