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29688-U

Supelco

SP®-2560 Capillary GC Column

L × I.D. 200 m × 0.25 mm, df 0.20 μm

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About This Item

UNSPSC Code:
12352211
NACRES:
SB.54

material

fused silica

Quality Level

100

Agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

313

df

0.20 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

200 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
262350-04
262350-03
262350-02
262350-01
257444-02

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W M Nimal Ratnayake et al.
Journal of AOAC International, 85(5), 1112-1118 (2002-10-11)
This study examines the effect of the column operating temperature of 100 m SP-2560 and CP-Sil 88 capillary gas chromatographic (GC) columns on the separation of cis- and trans-octadecenoic (18:1) isomers in partially hydrogenated vegetable oils. The overlapping GC peaks
R R Gangidi et al.
Lipids, 39(6), 577-582 (2004-11-24)
Conjugated linoleic acid (CLA), an anticarcinogenic compound with numerous other health benefits, is present mainly in dairy and beef lipids. The main CLA isomer present in dairy and beef lipids is cis 9, trans 11 CLA at a 0.5% concentration.
Pierluigi Delmonte et al.
Journal of chromatography. A, 1218(3), 545-554 (2010-12-24)
The ionic liquid SLB-IL111 column, available from Supelco Inc., is a novel fused capillary gas chromatography (GC) column capable of providing enhanced separations of fatty acid methyl esters (FAMEs) compared to the highly polar cyanopropyl siloxane columns currently recommended for
Shiping Shao et al.
Se pu = Chinese journal of chromatography, 33(11), 1214-1218 (2016-03-05)
A method to determine docosahexenoic acid (DHA) in milk powder by gas chromatography was established. The milk powder samples were hydrolyzed with hydrochloric acid, extracted to get total fatty acids by Soxhlet extractor, then esterified with potassium hydroxide methanol solution
Carla Ragonese et al.
Analytical chemistry, 81(13), 5561-5568 (2009-06-02)
The present research is focused on the evaluation of one 0.10 mm i.d. and two 0.25 mm i.d., ionic liquid (IL) stationary phase [1,9-di(3-vinyl-imidazolium) nonane bis(trifluoromethyl) sulfonyl imidate] columns, with lengths of 12 (the microbore capillary), 30 and 100 m
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Articles

200 Meter GC Columns for Detailed Analysis of cis/trans FAME Isomers

The SP-2560/SLB-IL111 column pairing allows for comprehensive and accurate (qualitative and quantitative) determinations of saturated and trans fatty acids.

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