ZRB2152

Anti-STAT5B Antibody, clone 2G13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Signal transducer and activator of transcription 5B
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2G13, recombinant monoclonal
• description: 2G13 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 89.87 kDa; observed mol wt ~80 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey, bovine, feline, porcine, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_036580
• UniProt accession no.: P51692
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... STAT5B(6777)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2G13 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects STAT5B. It targets an epitope within 20 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human STAT5B.

Application

Quality Control Testing

Evaluated by Western Blotting in MOLT4 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected STAT5B in MOLT4 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected STAT5B in lysate from Daudi cells and 1:1,000 dilution in lysate from HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected STAT5B in human placenta and human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound STAT5B peptide with a KD of 1.0 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected STAT5B in Daudi cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Signal transducer and activator of transcription 5B (UniProt: P51692; also known as STAT5B) is encoded by the STATB gene (Gene ID: 6777) in human. STAT (Signal Transducers and Activators of Transcription) proteins are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and serve as principal substrates of Janus kinases (JAK). In resting cells, STATs exist in an inactive state in the cytoplasm until phosphorylated and activated. Activators include cytokines, growth factors, and some peptides. STAT5B carries out the dual function of signal transduction and activation of transcription. It mediates cellular responses to the KIT ligand/SCF and other growth factors. Binds to the -interferon activation site (GAS) element and activates PRL-induced transcription. It is known to positively regulate hematopoietic/erythroid cell differentiation. Following tyrosine phosphorylation in response to KIT signaling, it is activated and translocates to the nucleus. It can also be phosphorylated by JAK2. Its phosphorylation at tyrosine 699 by protein tyrosine kinase 6 (PTK6) or Hematopoietic cell kinase (HCK) leads to an increase in its transcriptional activity. Mutations in STAT gene have been linked to large granular lymphocytic (LGL) leukemia. The Y665F and N642H mutants show increased transcriptional activity and tyrosine 694 phosphorylation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rajala, HLM., et al. (2013). Blood. 121(22); 4541-4550; Kanai, T., et al. (2012). Front. Immunol. 3; 234).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable