ZRB1672

Anti-S100-A8 Antibody, clone 1F16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Leukocyte L1 complex light chain, MRP-8, S100 calcium-binding protein A8, p8
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F16, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 10.84 kDa; observed mol wt ~10 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001306130
• UniProt accession no.: P05109
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... S100A8(6279)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F16 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Protein S100-A8. It targets an epitope within 12 amino acids within the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Protein S100-A8.

Application

Quality Control Testing

Evaluated by Western Blotting in Human peripheral blood mononuclear cells (PBMC) lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S100-A8 in lysate from peripheral blood mononuclear cells (PBMC).

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected S100-A8 in THP-1 and HL-60 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound S100-A8 peptide with a KD of 5.7 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected S100-A8 in human spleen tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected S100-A8 in one million Human peripheral blood mononuclear cells (PBMC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Protein S100-A8 (UniProt: P05109; also known as MRP-8, Migration inhibitory factor-related protein 8 Calgranulin-A, Calprotectin L1L subunit, Leukocyte L1 complex light chain, p8, S100 calcium-binding protein A8) is encoded by the S100A8 (also known as MRP8) gene (Gene ID:  6279) in human. Protein S100-A8 is a calcium- and zinc-binding proteins that plays a prominent role in the regulation of inflammatory processes and immune response. It is known to induce neutrophil chemotaxis and adhesion. It is predominantly found as calprotectin and performs a variety of intra- and extracellular functions. Its intracellular functions include facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes, and activation of the neutrophilic NADPH-oxidase. Protein S100-A8 activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane. It contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. Its extracellular functions include pro-inflammatory, antimicrobial, oxidant-scavenging, and induction of apoptosis. MRP-8 also acts as danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end (AGER) products. It also plays an impotent role as oxidant scavengers and has a protective role in preventing exaggerated tissue damage by scavenging oxidants. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Atallah, M., et al. (2012). PloS One. 7(2); e29333; Nakatani, Y., et al. (2005). Mediators Inflamm. 2005(5); 280-292; Ryckman, C., et al. (2003). J. Immunol. 170(6); 3233-3242).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable