ZRB1671

Anti-IgG1 Fc γ chain Antibody, clone 1B17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, Immunoglobulin heavy constant gamma 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1B17 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 43.9 kDa; observed mol wt ~46 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: N/A
• UniProt accession no.: P01857
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... IGHG1(3500)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects human IgG1 Fc chain. It targets an epitope within 16 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human IgG1 Fc chain.

Application

Quality Control Testing

Evaluated by Western Blotting with Human IgG, Fc fragment.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected Human IgG1 Fc chain.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot did not detect Mouse IgG, Rabbit IgG, and Chicken IgY.

Flow Cytometry Analysis: 1 µg from a representative lot detected IgG1 Fc chain in one million Raji cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IgG1 Fc chain in Human tonsil tissue sections.

Enzyme Immunoassay (ELISA): Serial dilutions from a representative lot detected Human IgG1 Fc fragment, but did not detect Mouse IgG and Chicken IgY.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Immunoglobulin heavy constant gamma 1 (UniProt: P01857; also known as Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE) is encoded by the IGHG1 gene (Gene ID: 3500) in human. IgG1 Fc belongs to the constant region of immunoglobulin heavy chains. It enables antigen binding activity and immunoglobulin receptor binding activity and acts upstream of or within several processes, including antibody-dependent cellular cytotoxicity, phagocytosis, and positive regulation of immune response. Two isoforms of IgG1 Fc have been described that are produced by alternative splicing. Isoform 1 is a secreted form and isoform 2 is a single-pass membrane protein with one extracellular domain (aa 1-350), a transmembrane domain (aa 351-371), and a cytoplasmic domain (aa 372-399). IgG1 Fc contains three Ig-like domains (aa 6-99; 121-220; and 229-325) that are localized in the extracellular domain. It is reported to mediate IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells. Its glycosylation on asparagine 180 is shown to be essential for interaction with Fc receptors and ability to activate complement pathway. Over-expression of IGHG1 has been linked to enhanced proliferation of gastric cancer cells via AKT/GSK-3β/β-Catenin pathway. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, X., et al. (2021). Cancer Cell Int. 21; Article 397; Allhorn, M., et al. (2010). Blood. 115(24); 5080-5088).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.