Z4751

Anti-Zyxin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-ESP-2, Anti-HED-2
• MDL number: MFCD01634364
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 82-84 kDa
• species reactivity: mouse, human, rat
• technique(s): indirect immunofluorescence: 1:100 using Rat1 cells; western blot (chemiluminescent): 1:1,000 using whole extract of human HeLa cells; western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 cells
• UniProt accession no.: Q15942
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ZYX(7791) ; mouse ... Zyx(22793) ; rat ... Zyx(114636)

Description

General description

Zyxin (ZYX ), a zinc-binding phosphoprotein of ∼61kDa is located at focal adhesions and along the actin cytoskeleton. It has three LIM domains. This structural protein is located on human chromosome 7q34.

Immunogen

synthetic peptide corresponding to amino acid residues 134-150 of human zyxin with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse.

Application

Anti-Zyxin antibody has been used in fluorescence microscopy.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 papers)

Biochem/physiol Actions

Zyxin (ZYX ) participates in the modulation of actin assembly at focal adhesions and stress fibers. It also regulates cell adhesion and cell cycle. Overexpression of ZYX is associated with melanoma. Suppression of ZYX  prevents cell growth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves