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Key Documents

V4757

Sigma-Aldrich

Nalgene® cryogenic vials

capacity 1.2 mL, External threads, sterile; irradiated

Synonym(s):

cryostorage vial, cryovial cryotube

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About This Item

UNSPSC Code:
41121704
NACRES:
NB.11

material

colorless tube
conical bottom
high-density polyethylene closure
polypropylene
polypropylene tube

sterility

sterile; irradiated

feature

External threads

packaging

package of 25 vials x 20
pack of 500 ea (20 x 25ea)

manufacturer/tradename

Nalgene® 5000-0012

parameter

-150 °C min. temp.

technique(s)

cryopreservation: suitable

capacity

1.2 mL

tube diam.

12.7 mm

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General description

Vials designed for exceptional ease of use and sample integrity in ultra-low temperature storage. Closure seals without gasket or O-ring, minimizing risk of contamination. The deep skirt of the closure allows single-handed aseptic technique without exposing fingers to vial contents. Ribs around the bottom of the vial interlock with wells in the Cryovial Racks so the vial will not turn when opening or closing. Bottoms of vials are skirted; vial stands without holder.

Sterile vials and closures are pre-assembled and radiation-sterilized. A white marking area, fill line and graduations are printed on the side. Certified sterile, non-cytotoxic and non-pyrogenic.

Note: Do not use cryogenic vials for storage in the liquid phase of liquid nitrogen unless correctly sealed in Nunc CryoFlex™ tubing. Such use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up resulting in possible explosion or biohazard release.

Legal Information

CryoFlex is a trademark of Thermo Fisher Scientific or its subsidiaries
Nalgene is a registered trademark of Thermo Fisher Scientific or its subsidiaries

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

CryoStor® Cryopreservation Protocol

Cryopreservation efficacy which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis.

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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