SRP3147

Relaxin-2 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): H2 relaxin, RLN2
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: 6.0 kDa
• packaging: pkg of 25 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: P04090
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... RLN2(6019)

Description

General description

Relaxin-2 is a peptide hormone structurally related to insulin. The gene is mapped to human chromosome 9p24. It is expressed in the placenta, decidua, prostate, corpus luteum, endometrium, mammary glands, heart, and brain. It is also expressed in the ovary during pregnancy. Recombinant relaxin-2 is a nonglycosylated 6kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 29 amino acid B-chain.

Application

It has also been used to study its effect on human endometrial carcinoma cell lines.

Relaxin-2 human has been used to study the effect of testosterone on the expression of relaxin receptor in the knee joint.

Biochem/physiol Actions

Of the three known relaxin genes, Relaxin-2 (RLN2) is the only relaxin known to circulate in the blood. Relaxin-2 binds specifically to the LGR7 (leucine-rich repeat-containing G-protein coupled receptor 7) and LGR8 receptors, previously identified as an "orphan" G protein coupled receptors. Signaling by Relaxin-2 through its target receptors enhances the growth of pubic ligaments and ripening of the cervix during birth. Relaxin-2 also participates in cancer progression. In prostate cancer, it is responsible for tumor growth and vascularisation. Relaxin-2 is also linked with anti-fibrotic and cardioprotective effects.

Sequence

A-Chain: QLYSALANKC CHVGCTKRSL ARFC B-Chain: DSWMEEVIKL CGRELVRAQI AICGMSTWS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable