SRP2031

CTF1 human

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

• Synonym(s): CTF, CTF/NFI, NF-1, NF-I
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in insect cells
• Assay: ≥70% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~56.4 kDa
• packaging: pkg of 5 μg
• concentration: 500 μg/mL
• color: colorless to clear
• NCBI accession no.: X12492
• UniProt accession no.: Q16619
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CTF-1(1489)

Description

Biochem/physiol Actions

The CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator. It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes. Cloning and functional characterization of the protein revealed that CTF1 contains an N-terminal DNA binding domain and a C-terminal proline-rich activation domain. CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes, with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene, as well as other receptor proteins to synergize hormone receptor-responsive activation.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable