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Key Documents

SRP0106

Sigma-Aldrich

HDAC-5 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

HD5, Histone deacetylase 5, NY-CO-9

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥80% (SDS-PAGE)

form

aqueous solution

potency

≥1 units/mg

mol wt

51 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_001015053

UniProt accession no.

Q9UQL6

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HDAC5(10014)

General description

Human HDAC5, catalytic domain, GenBank Accession No. NM_001015053, amino acid 657-1123 with C-terminal His tag, MW = 51 kDa, expressed in baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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