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S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

form

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Application

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Features and Benefits

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

related product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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A J Bogers et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 2(3), 160-166 (1988-01-01)
To identify risk factors for hospital mortality after postinfarction left ventricular aneurysmectomy (PILVA), 83 consecutive cases of PILVA were studied in a retrospective analysis. Hospital mortality was 14.4%. Most of the factors correlated with hospital mortality were associated with poor
Aline V Probst et al.
Developmental cell, 19(4), 625-638 (2010-10-19)
At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how
Christèle Maison et al.
Nature genetics, 43(3), 220-227 (2011-02-15)
HP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of

Protocols

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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