S5772

Sephadex^® G-25

BioReagent, for molecular biology, DNA grade, Superfine

• CAS Number: 9041-35-4
• MDL number: MFCD00132770
• UNSPSC Code: 23151817
• NACRES: NA.52

Properties

• grade: DNA grade; for molecular biology
• product line: BioReagent
• form: beads
• technique(s): buffer exchange: suitable
• matrix active group: phase
• bead diameter: 20-50 μm
• pore size: exclusion limit (10 bp dsDNA; (rA)10)
• pH range: 2-13
• application(s): life science and biopharma
• compatibility: Cytiva
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• InChI: 1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
• InChI key: BCPVZFFJGCURNR-OCOFDJSDSA-N

Description

General description

Sephadex^® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex^® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Application

Sephadex^® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves