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Key Documents

RAB0675

Sigma-Aldrich

Human Hemoglobin ELISA Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

technique(s)

ELISA: suitable

assay range

inter-assay cv: <10%
intra-assay cv: <12%

shipped in

wet ice

storage temp.

−20°C

General description

The antibody pair provided in this kit recognizes Human Hemoglobin. Hemoglobin (Hb) is an iron-containing metalloprotein, which is highly expressed in red blood cells. In addition, it is also expressed in nonerythroid cells such as epithelial cells of different origin. Hb is a tetramer composed of two α-globin chains and two β-globin chains. α-globin and β-globin chains is encoded by a gene mapped to human chromosome 16 and 11, respectively.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Hemoglobin (Hb) serves as the primary means of oxygen transport in vertebrates. Hb exhibits antioxidant and antimicrobial activity. Alterations in blood hemoglobin levels occur in many diseases such as anemia and polycythemia. In addition, abnormalities in Hb concentration might be implicated in pathologies associated with various respiratory diseases, such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive lung disease (COPD).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Polycythemia vera masked due to severe iron deficiency anemia.
Kambali S and Asma T
Hematology/oncology and Stem Cell Therapy, 11(1), 38-40 (2016)
Mechanisms linking red blood cell disorders and cardiovascular diseases.
Mozos I.
BioMed Research International, 2015, 12-12 (2015)
Hemoglobin expression in nonerythroid cells: novel or ubiquitous?
Saha D, et al.
International Journal of Inflammation, 2014, 8-8 (2014)

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