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PP0540

Sigma-Aldrich

GlycoProfile™ β-Elimination Kit

Sufficient for 24 samples

Synonym(s):

β-Elimination Glycan Kit, Glycan Removal Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.28

conjugate

(O-linked)

Quality Level

200

storage temp.

room temp

Application

The GlycoProfile™ β-Elimination kit contains a novel non-reducing reagent to efficiently and specifically remove O-linked carbohydrates from glycoproteins with minimal protein or glycan destruction. Consequently, additional downstream proteomics and glycomic analyses may be employed.

Quantity

The kit includes the reagents and techware for O-deglycosylation of up to 24 samples (200 uL at 1-10 ug/uL of glycoprotein per sample).

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Sodium hydroxide solution, 5.0 M 60 μL
  • Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea

Kit Components Also Available Separately

Product No.
Description
SDS
  • Centrifugal Filter Unit

Pictograms

FlameCorrosion
GHS02,GHS05

Signal Word

Danger

Hazard Statements

H226,H290,H314

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

3 - Flammable liquids

Flash Point(F)

82.0 °F - closed cup

Flash Point(C)

27.79 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)

Articles

GlycoProfile™ ß-Elimination Kit

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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