P4039

Phospholipase C from Clostridium perfringens (C. welchii)

Type XIV, lyophilized powder, ≥150 units/mg protein

• Synonym(s): Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
• CAS Number: 9001-86-9
• Enzyme Commission number: 3.1.4.3 (BRENDA, IUBMB)
• EC Number: 232-638-2
• MDL number: MFCD00131895
• UNSPSC Code: 41106300
• NACRES: NA.32

Properties

• biological source: bacterial (Clostridium perfringens)
• Quality Level: 200
• type: Type XIV
• form: lyophilized powder
• specific activity: ≥150 units/mg protein
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• greener alternative category: Enabling,
• storage temp.: −20°C
• Gene Information: Clostridium perfringens str. 13 ... plc(988262)

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Application

Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.

Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder in buffered salts

Preparation Note

Chromatographically purified

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves