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Key Documents

P2938

Sigma-Aldrich

Immobilon®-P Polyvinylidene difluoride membranes

size 26.5 cm × 375 cm

Synonym(s):

PVDF

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

manufacturer/tradename

Millipore IPVH00010

Quality Level

size

26.5 cm × 375 cm

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Application

Immobilon-P Polyvinylidene difluoride membrane has been used in western blotting for the transfer of proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).
Polyvinylidene difluoride membranes are popular membranes for immunoblotting (Western blotting), and sequencing of proteins. The basis of protein binding to PVDF is believed to be due to hydrophobic interactions. PVDF membranes for Western blotting are available in the most commonly used 0.45 μm pore size. PVDF membranes have a greater protein binding capacity, are more durable and more solvent resistant than nitrocellulose.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cross-phosphorylation between Arabidopsis thaliana sucrose nonfermenting 1-related protein kinase 1 (AtSnRK1) and its activating kinase (AtSnAK) determines their catalytic activities.
Crozet P, et al.
The Journal of Biological Chemistry, 285, 12071-12077 (2010)
Clare M Hamilton et al.
Infection and immunity, 77(6), 2488-2498 (2009-04-01)
Parasitic worms and molecules derived from them have powerful anti-inflammatory properties and are shown to have therapeutic effects on inflammatory diseases. The helminth Fasciola hepatica has been reported to suppress antigen-specific Th1 responses in concurrent bacterial infections, thus demonstrating its

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