N5254

Neuraminidase Agarose from Clostridium perfringens (C. welchii)

Type VI-A, ammonium sulfate suspension

• Synonym(s): Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• MDL number: MFCD00131711
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type VI-A
• form: ammonium sulfate suspension
• specific activity: 20-60 units/g agarose
• extent of labeling: 0.6-1.8 units per mL gel; 20-60 units per g agarose
• matrix: beaded agarose
• storage temp.: 2-8°C

Description

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Agarose-linked neuraminidase from Clostridium perfringens can be used for the detection of total and desialylated sex hormone-binding globulin in human serum samples.

Neuraminidase from Clostridium perfringens has been used in a study to assess a genetic polymorphism of human serum glycoprotein (inter-α-trypsin-inhibitor). It has also been used in a study to investigate the inhibition of neuraminidase by chemically sulphated glycopeptides.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Suspension in 2.0 M (NH₄)₂SO₄ solution, pH 7.0.

Preparation Note

Prepared from Neuraminidase, Type VI (N 3001).

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable