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MAK021

Sigma-Aldrich

High Sensitivity Glucose-6-Phosphate Assay Kit

sufficient for 100 fluorometric tests

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About This Item

EC Number:
222-938-1
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

cancer; hematological disorder

storage temp.

−20°C

General description

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high. G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. The Glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for quantifying Glucose-6-Phosphate concentrations in a variety of samples.

Principle

Glucose-6-phosphate concentration is by a enzyme assay, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the G6P present. Typical sensitivities of detection for this kit are between 100-500 pmoles. The Glucose-6-Phosphate Assay Kit is much more sensitive than the Glucose-6-Phosphate Colorimetric Assay Kit (MAK014) and is suitable for samples with low concentrations of glucose-6-phosphate.

replaced by

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Role of the liver in the control
of carbohydrate and lipid homeostasis
Postic C, et al.
Diabete & Metabolisme, 30, 398-408 (2004)
Glucose 6-phosphate dehydrogenase deficiency: from genotype to phenotype.
Lucio L.
The Hematology Journal : The Official Journal of the European Haematology Association / EHA, 1303-1306 (2006)
Glucose-6-phosphate dehydrogenase, NADPH, and cell survival.
Robert C S.
IUBMB Life, 64(5), 362-369 (2012)
Qiuhua Yang et al.
Nature communications, 9(1), 4667-4667 (2018-11-09)
Increased aerobic glycolysis in endothelial cells of atheroprone areas of blood vessels has been hypothesized to drive increased inflammation and lesion burden but direct links remain to be established. Here we show that endothelial cells exposed to disturbed flow in

Articles

Aerobic Glycolysis and the Warburg Effect

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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