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M3546

Sigma-Aldrich

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)

Synonym(s):

protein markers, protein molecular weight markers, protein standards

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About This Item

UNSPSC Code:
41105337
NACRES:
NA.25

usage

 vial sufficient for 400 applications on large gels (16 × 18 cm)
 vial sufficient for 800 mini-gels (10 × 10 cm)

packaging

vial of 200 μL

storage temp.

−20°C

Application

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600) has been used as a protein marker in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot.

Biochem/physiol Actions

The ultralow range marker is designed for use in the Tris-Tricine SDS-PAGE method when stained with Brilliant blue G (non-methanol based). Glutaraldehyde fixing is recommended with the ultralow marker in order to keep the two smallest proteins fixed in the gel. The ultralow range marker is supplied as a liquid, which must be diluted (1:20) with the supplied sample buffer prior to loading.

Physical form

Solution in 0.1 M Tris-HCl, pH approx. 8.5, with 4 mM EDTA, 3 mM sodium azide and 40% glycerol

Signal Word

Danger

Hazard Statements

H312,H317,H318,H361d,H412

Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Endostatin enhances radioresponse in breast cancer cells via alteration of substance P levels.
Arslan Aydemir E, et al.
Oncology Letters, 2, 879-886 (2011)
Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system.
Morassutti C, et al.
Febs Letters, 519, 141-146 (2002)
Christelle Richard et al.
Applied and environmental microbiology, 70(1), 248-254 (2004-01-09)
Polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized C-terminal part of divercin V41 (DvnCt) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and reactivity of the DvnCt-KLH-generated antibodies were evaluated by enzyme-linked
Raquel Soares Oliveira et al.
Toxins, 12(6) (2020-06-19)
The biological activity of Rhinella icterica parotoid secretion (RIPS) and some of its chromatographic fractions (RI18, RI19, RI23, and RI24) was evaluated in the current study. Mass spectrometry of these fractions indicated the presence of sarmentogenin, argentinogenin, (5β,12β)-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide, marinobufagin, bufogenin
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations

Protocols

HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)

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