M2414

Minimum Essential Medium Eagle

With Earle′s salts and reduced NaHCO₃. without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): EMEM, MEM
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Minimum Essential Medium Eagle, Modified, with Earle′s salts and reduced NaHCO₃. without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: 0.011 g/L; NaHCO₃: 0.85 g/L; glucose: 1.0 g/L; L-glutamine: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application

Recommended for use in a carbon dioxide-free culture system.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln.

May also be supplemented with HEPES to raise the buffering range.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable