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L1668

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

Quality Level

200

product line

BioReagent

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

storage temp.

2-8°C

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Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for the stimulation of cluster of differentiation (CD4+) T cells
  • for the activation of primary peripheral blood lymphocytes
  • for the stimulation of peripheral blood mononuclear cells

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

Sold on the basis of lectin content

Analysis Note

Potency and solubility expressed in mg lectin/ml

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Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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